RNA-seq of soil: pasture sodosol - SRA

SRX22006854: RNA-seq of soil: pasture sodosol
1 ILLUMINA (Illumina NovaSeq 6000) run: 98.7M spots, 29.6G bases, 9.2Gb downloads

Design: Soil samples were collected in December 2021 from the University of Sydney-owned Nowley Farm on the Liverpool plains, New South Wales, Australia. Samples were packed into sterile 50 ml conical tubes and stored on ice until transported to a -80C freezer for long-term storage. Total RNA was extracted from the samples using the RNeasy PowerSoil Total RNA Kit (Qiagen) as per the manufacturers instructions. Libraries were constructed using the Illumina Stranded Total RNA library preparation protocol and rRNA was removed using the Ribo-Zero Plus rRNA depletion kit (Illumina). Libraries were sequenced on the Illumina NovaSeq 6000 platform (paired-end, 150 bp). Library preparation and sequencing was performed by the Australian Genome Research Facility (AGRF). Data generated as part of the study Australian terrestrial environments harbour extensive RNA virus diversity.

Submitted by: The University of Sydney

Study: Australian terrestrial environments harbour extensive RNA virus diversity

PRJNA981585 • SRP464815 • All experiments • All runs

show Abstracthide Abstract

Meta-transcriptomic sequencing of Australian soil and sediment samples reveal remarkable viral diversity, reflecting the diversity of Australian plants and soil-dwelling invertebrates and microbes.

Sample:

SAMN35681321 • SRS19081518 • All experiments • All runs

Organism: soil metagenome

Library:

Name: SP2A

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: METATRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 98.7M spots, 29.6G bases, 9.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR26298330	98,665,119	29.6G	9.2Gb	2024-02-21

ID:: 29920067

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